Serotonin activates Cl- channels in the apical membrane of rat choroid plexus epithelial cells

The effects of serotonin on ion channel activity in epithelial cells from rat choroid plexus were examined. Serotonin (50 nM, 500 nM and 1 microM) stimulated channel activity in cell-attached patches. The current-voltage (I-V) relationship for the serotonin-activated channel gave a conductance of 26.6 +/- 1.5 pS and the current reversed at an applied electrode potential (-Vp) = 15.3 +/- 3.3 mV with a KCl-rich electrode solution (n = 8). Similar I-V relationships were obtained using electrode solutions in which K+ was replaced by other cations (Na+ and N-methyl-D-glucamine), suggesting that the serotonin-activated channels are selective to Cl-. The effect of 1 microM serotonin on channel activity was inhibited by ritanserin (30 and 100 nM) which has a high affinity for serotonin 5-HT1C receptors and 5-HT2 receptors. Spiperone (30 nM), which binds weakly to 5-HT1C receptors but has a high affinity for 5-HT2 receptors, did not inhibit the actions of serotonin. These data suggest that serotonin increases Cl- channel activity by acting on the 5-HT1C receptors on the epithelium.