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The comparison of DNA synthesis inducibility by high potassium treatment in various nonproliferating cells
Y V Fedorov1, I A Prudovsky, Y E Yegorov
1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Abstract:
Previous studies have demonstrated that treatment of fibroblasts from confluent, density-inhibited cultures with 50 mM KCl solution led to the onset of DNA replication. In the present study we show that such treatment can induce aphidicolin-sensitive DNA replication in differentiated nondividing cells: dorsal root ganglia neurons and in vitro differentiated myotubes formed by L6 myoblasts. Murine peritoneal macrophages and macrophages/granulocytes derived from human promyelocytic leukemia cells HL60 are refractory to high potassium treatment. These results confirm the nonuniformity of the nonproliferative state of various differentiated cells.
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