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No enzymatic activities are necessary for the stabilization of ascorbic acid by K-562 cells
E Schweinzer1, G Waeg, H Esterbauer
1Department of Medical Chemistry, University of Vienna, Wien, Austria.
FEBS Letters
|November 8, 1993
Abstract:
We disprove that living cells stabilize ascorbate by the activity of a trans plasma membrane semidehydroascorbate reductase. The two processes show different specificities for both substrate and inhibitor. Not only cells but also cell-conditioned buffers stabilize ascorbate as long as compounds with molecular weights above 10 kDa are not removed. The effect is most probably due to chelation of traces of transition metals.