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Interleukin-10 inhibits endotoxin-induced tissue factor mRNA production by human monocytes

M Ramani1, V Ollivier, F Khechai

  • 1INSERM U294, CHU Xavier Bichat, Paris, France.

FEBS Letters
|November 8, 1993
PubMed

Insights

Interleukin-10 (IL-10) suppresses endotoxin-induced tissue factor (TF) in monocytes, suggesting its therapeutic potential for Gram-negative septic shock. This finding could help reduce mortality rates associated with this severe condition.

Area of Science:

  • Immunology
  • Hematology
  • Critical Care Medicine

Background:

  • Gram-negative septic shock involves human monocytes expressing tissue factor (TF), a key coagulation activator.
  • TF is implicated in triggering disseminated intravascular coagulation (DIC) during septic shock.

Purpose of the Study:

  • To investigate the effect of interleukin-10 (IL-10) on endotoxin-induced TF activity and expression in human monocytes.
  • To explore the potential therapeutic role of IL-10 in managing septic shock.

Main Methods:

  • Monocytes were stimulated with endotoxin.
  • TF activity, antigen levels, and mRNA expression were measured.
  • The impact of IL-10 treatment on these parameters was assessed.

Main Results:

  • IL-10 significantly suppressed endotoxin-induced TF activity and antigen levels in monocytes.
  • IL-10 also reduced the expression of TF mRNA in response to endotoxin.

Conclusions:

  • IL-10 demonstrates a suppressive effect on TF induction in monocytes during endotoxin challenge.
  • These findings highlight IL-10 as a potential therapeutic agent for septic shock, aiming to mitigate DIC and improve patient outcomes.

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