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Stimulation of plasmin activity in cultured human fibroblast cells by Porphyromonas endodontalis
1Department of Oral Surgery, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Abstract:
1. Plasmin activity in the conditioned medium of Gin-1 cells, a human gingival fibroblast cell line, was stimulated by Porphyromonas endodontalis, a putative pathogen of oral submucous abscesses, in a time- and dose-dependent manner. 2. P. endodontalis stimulated the activity of plasminogen activator in both the conditioned medium and the cell lysate. The plasminogen activator in Gin-1 cells was approx. 50 kDa by zymography. 3. The conditioned medium of Gin-1 cells exposed to P. endodontalis stimulated the conversion of human serum prekallikrein to kallikrein. 4. These results suggested that P. endodontalis stimulates the plasminogen activator-plasmin system in Gin-1 cells, and that activated plasmin plays a role in the progress of periodontal tissue inflammation.
Insights
Porphyromonas endodontalis infection stimulates plasminogen activator activity in human gingival fibroblasts. This activation contributes to periodontal tissue inflammation, highlighting a key mechanism in oral submucous abscesses.
Area of Science:
- Oral microbiology
- Biochemistry
- Periodontology
Background:
- Porphyromonas endodontalis is implicated in oral submucous abscesses.
- Fibroblast activation of the plasminogen system is crucial in tissue remodeling and inflammation.
Purpose of the Study:
- To investigate the effect of P. endodontalis on plasmin activity in human gingival fibroblasts (Gin-1 cells).
- To elucidate the role of the plasminogen activator-plasmin system in P. endodontalis-induced inflammation.
Main Methods:
- Zymography was used to analyze plasminogen activator activity.
- Gin-1 cells were exposed to P. endodontalis.
- Prekallikrein conversion was measured in conditioned medium.
Main Results:
- P. endodontalis stimulated plasmin activity in a time- and dose-dependent manner.
- Plasminogen activator activity was increased in both conditioned medium and cell lysate.
- Activated plasmin contributes to the progression of periodontal tissue inflammation.
Conclusions:
- P. endodontalis activates the plasminogen activator-plasmin system in human gingival fibroblasts.
- Activated plasmin plays a significant role in periodontal tissue inflammation associated with oral submucous abscesses.