Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium channel blockers apparently decrease noradrenaline release from nerve-skin terminals in Caudiverbera

B Norris1, J Concha, G Contreras

  • 1Department of Physiology, Faculty of Biological Sciences, University of Concepción, Chile.

General Pharmacology
|July 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Flp-recombinase mouse line for genetic manipulation of ipRGCs.

bioRxiv : the preprint server for biology·2024
Same author

Assessment of seasonal and annual patterns in phosphorus content in a monitored catchment through a partitioning approach based on hydrometeorological data.

Environmental research·2023
Same author

Accounting for the annual variability when assessing non-point source pollution potential in Mediterranean regulated watersheds.

The Science of the total environment·2023
Same author

ICARUS at the Fermilab Short-Baseline Neutrino program: initial operation.

The European physical journal. C, Particles and fields·2023
Same author

Identifying the required degree of disease clearance to improve quality of life in pemphigus vulgaris.

The British journal of dermatology·2020
Same author

Aggressive behavior in cattle is associated with a polymorphism in the MAOA gene promoter.

Animal genetics·2019
Same journal

Endothelium-dependent vasorelaxant and antiproliferative effects of apigenin.

General pharmacology·2002
Same journal

Reversal of hypercapnia induces endothelin-dependent constriction of basilar artery in rabbits with acute metabolic alkalosis.

General pharmacology·2002
Same journal

Reversal of hypercapnia induces KATP channel and NO-independent constriction of basilar artery in rabbits with acute metabolic alkalosis.

General pharmacology·2002
Same journal

Contractile responses in spontaneously diabetic mice. II. Effect of cholestyramine on enhanced contractile response of aorta to norepinephrine in C57BL/KsJ (db/db) mice.

General pharmacology·2002
Same journal

Contractile responses in spontaneously diabetic mice. I. Involvement of superoxide anion in enhanced contractile response of aorta to norepinephrine in C57BL/KsJ(db/db) mice.

General pharmacology·2002
Same journal

Investigation of basal endothelial function in the obese Zucker rat in vitro.

General pharmacology·2002
See all related articles

Three calcium channel blockers, verapamil, diltiazem, and nifedipine, were found to reduce noradrenaline release at the skin neuroepithelial synapse in the toad Caudiverbera caudiverbera. These blockers dose-dependently inhibited responses to electrical stimulation.

Area of Science:

  • Neuropharmacology
  • Amphibian Physiology
  • Synaptic Transmission

Background:

  • The skin neuroepithelial synapse plays a role in physiological responses.
  • Calcium channels are critical for neurotransmitter release.
  • Understanding the role of calcium in this synapse is important.

Purpose of the Study:

  • To investigate the effects of calcium channel blockers on the toad skin neuroepithelial synapse.
  • To determine the role of calcium influx in mediating responses to electrical stimulation.
  • To elucidate the mechanism of noradrenaline release at this synapse.

Main Methods:

  • Electrical stimulation of the toad skin neuroepithelial synapse.
  • Application of calcium channel blockers (verapamil, diltiazem, nifedipine) in a dose-dependent manner.

Related Experiment Videos

  • Measurement of potential difference (PD) and short-circuit current (SCC).
  • Assessment of responses to noradrenaline and amiloride.

    • Main Results:

    • Electrical stimulation significantly increased PD and SCC.
    • Verapamil, diltiazem, and nifedipine dose-dependently reduced these responses, with verapamil showing the greatest effect.
    • Amiloride did not affect the responses, ruling out sodium channel involvement.
    • Noradrenaline-induced skin responses were unaffected by calcium channel blockers.

    Conclusions:

    • Calcium channel blockers verapamil, diltiazem, and nifedipine inhibit electrical stimulation-induced responses in the C. caudiverbera skin neuroepithelial synapse.
    • These findings suggest that calcium influx through voltage-gated channels is essential for the release of noradrenaline at this synapse.
    • The study highlights the critical role of calcium in regulating neurotransmitter release in amphibian skin.