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Pathological activities of Yersinia ruckeri, the enteric redmouth (ERM) bacterium

J L Romalde1, A E Toranzo

  • 1Departamento de Microbiología y Parasitología, Facultad de Biología, Universidad de Santiago de Compostela, Spain.

FEMS Microbiology Letters
|September 15, 1993
PubMed

Insights

Yersinia ruckeri exhibits moderate adhesion and invasiveness in salmon cells. Its extracellular products (ECP) are toxic to fish and possess various enzymatic and hemolytic activities, though heat treatment neutralizes most.

Area of Science:

  • Microbiology
  • Fish Pathology
  • Bacteriology

Background:

  • Yersinia ruckeri is an important fish pathogen.
  • Understanding its virulence factors is crucial for fish health management.

Purpose of the Study:

  • To investigate the adherence, invasiveness, and pathobiological activities of Yersinia ruckeri.
  • To characterize the extracellular products (ECP) of Yersinia ruckeri.

Main Methods:

  • Cell-line adhesion and invasion assays using salmon cell-line CHSE-214.
  • Toxicity and enzymatic activity assays of extracellular products (ECP).
  • SDS-PAGE analysis of lipopolysaccharide (LPS) and protein components.

Main Results:

  • All Yersinia ruckeri strains showed moderate adhesion and invasiveness in CHSE-214 cells.
  • ECP were highly toxic to fish (LD50: 2-9.12 µg protein/g fish) and exhibited caseinase, gelatinase, amylase, lipase, phospholipase, and hemolytic activities.
  • Heat treatment (100°C for 10 min) abolished most ECP activities, except gelatinase.
  • SDS-PAGE revealed strain-specific variations in LPS and protein components based on serotype.

Conclusions:

  • Yersinia ruckeri possesses significant adhesive and invasive potential against salmon cells.
  • The extracellular products of Yersinia ruckeri are potent virulence factors contributing to fish toxicity and enzymatic degradation.
  • Heat stability varies among ECP activities, with gelatinase being the most resistant.

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