Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A novel phage lambda replacement Cre-lox vector that has automatic subcloning capabilities

C L Holt1, G S May

  • 1Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.

Gene
|October 29, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The treatment of infectious hepatitis (catarrhal jaundice).

The Medical clinics of North America·2010
Same author

The value of the methylene blue test in the detection of bilirubin.

The New England journal of medicine·2010
Same author

Enhanced activity of strobilurin and fludioxonil by using berberine and phenolic compounds to target fungal antioxidative stress response.

Letters in applied microbiology·2007
Same author

Targeting antioxidative signal transduction and stress response system: control of pathogenic Aspergillus with phenolics that inhibit mitochondrial function.

Journal of applied microbiology·2006
Same author

Mitogen activated protein kinases of Aspergillus fumigatus.

Medical mycology·2005
Same author

Mutation in a calpain-like protease affects the posttranslational mannosylation of phosphatases in Aspergillus nidulans.

Fungal genetics and biology : FG & B·2003
Same journal

Hierarchical analysis of metabolic phenotype reveals distinct microbiota and circulatory transcriptome in metabolic dysfunction-associated steatotic liver disease.

Gene·2026
Same journal

Mutation T71R enhanced the structural stability and functional activity of wild type superoxide dismutase cloned from soil metagenome.

Gene·2026
Same journal

Reduced ATXN1 expression as an adverse prognostic indicator in Acute myeloid leukemia.

Gene·2026
Same journal

Constructing regulatory networks of Rubisco post-translational modifications: a novel avenue for engineering environment adaptive plants.

Gene·2026
Same journal

Traumatic brain injury enhances fracture healing by upregulating VNN1 to activate the Wnt/β-catenin signaling pathway.

Gene·2026
Same journal

Single-cell transcriptomics reveals CCL2-mediated macrophage-endothelial cell interactions drive apoptosis in varicose veins.

Gene·2026
See all related articles

Researchers created a new cloning vector, lambda pAn, for faster DNA analysis. It uses Cre-loxP recombination to automatically subclone inserts, simplifying genomic research and mutation cloning in Aspergillus nidulans.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Phage lambda replacement vectors are essential tools for cloning large DNA fragments.
  • Subcloning inserts from phage lambda vectors into plasmids can be a time-consuming and laborious process.
  • Efficient methods for genomic DNA analysis and mutation isolation are crucial in genetic research.

Purpose of the Study:

  • To develop a novel phage lambda replacement cloning vector, lambda pAn, that facilitates automated subcloning of inserts.
  • To enable rapid structural analysis of insert DNA and streamline the cloning process.
  • To provide a versatile tool for isolating genomic clones and studying mutations, particularly in Aspergillus nidulans.

Main Methods:

  • Development of the lambda pAn phage lambda replacement vector.

Related Experiment Videos

  • Utilized the Cre-loxP site-specific recombination system for automated subcloning.
  • Incorporated the pyrG gene of Aspergillus nidulans as a nutritional selective marker for transformation.
  • Tested the vector's capacity for inserts ranging from 5 to 19 kb.

    • Main Results:

    • The lambda pAn vector successfully enables automatic subcloning of inserts as plasmids via Cre-loxP recombination.
    • This eliminates the need for manual subcloning of insert fragments, significantly speeding up DNA analysis.
    • The vector accepts inserts in the 5 to 19 kb size range, comparable to existing replacement vectors.
    • The pyrG marker facilitates selection of transformed cells.

    Conclusions:

    • Lambda pAn is a novel and efficient phage lambda replacement vector that simplifies the cloning and structural analysis of DNA inserts.
    • The automated subcloning feature using Cre-loxP recombination offers a significant advantage over traditional methods.
    • This vector is particularly useful for isolating genomic clones and facilitating the study of mutations, such as dominant extragenic suppressor mutations in Aspergillus nidulans.