Jove
Visualize
Contact Us

Related Experiment Videos

New improved lacZ gene fusion vectors

C Jain1

  • 1Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.

Gene
|October 29, 1993
PubMed
Summary

New plasmid vectors were created for lacZ gene fusions, offering undetectable background beta-galactosidase activity and flexible cloning options. These improved vectors are available in medium- and high-copy-number formats for enhanced molecular biology applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Utility of serum Galactomannan in diagnosing COVID-19 patients with suspected IPA: an observational study in resource limited settings.

European review for medical and pharmacological sciences·2022
Same author

Corrigendum to 'Cardiovascular adverse events are associated with usage of immune checkpoint inhibitors in real-world clinical data across the United States': [ESMO Open Volume 6, Issue 5, October 2021, 100252].

ESMO open·2021
Same author

Cardiovascular adverse events are associated with usage of immune checkpoint inhibitors in real-world clinical data across the United States.

ESMO open·2021
Same author

Novel Coronavirus: What Neuroradiologists Should Do.

AJNR. American journal of neuroradiology·2020
Same author

Continental crust formation on early Earth controlled by intrusive magmatism.

Nature·2017
Same author

Nasopharyngeal non-intestinal-type adenocarcinoma: a case report and updated review of the literature.

Current oncology (Toronto, Ont.)·2017
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • LacZ gene fusions are essential tools in molecular biology for studying gene expression and protein localization.
  • Existing plasmid vectors for lacZ fusions have limitations, including background activity and restricted cloning flexibility.
  • Development of improved vectors is crucial for advancing research in various biological fields.

Purpose of the Study:

  • To develop novel plasmid vectors specifically designed for creating lacZ gene fusions.
  • To enhance the utility of lacZ fusion vectors by addressing limitations of current systems.
  • To provide researchers with versatile and efficient tools for molecular cloning and gene expression studies.

Main Methods:

  • Design and construction of new plasmid vectors incorporating an extended multiple cloning site (MCS).
  • Incorporation of features to ensure undetectable background beta-galactosidase (beta Gal) activity.
  • Development of subcloning capabilities for three distinct translational frames.
  • Generation of both medium- and high-copy-number versions of the developed vectors.

Main Results:

  • Successfully developed novel plasmid vectors for lacZ gene fusions.
  • Achieved undetectable background beta-galactosidase activity, improving signal-to-noise ratio.
  • Provided an extended MCS for greater cloning flexibility.
  • Enabled convenient subcloning into three translational frames.
  • Created both medium- and high-copy-number plasmid variants.

Conclusions:

  • The newly developed plasmid vectors offer significant improvements over existing systems for lacZ gene fusions.
  • These vectors provide enhanced performance and flexibility, facilitating more accurate and efficient gene expression studies.
  • The availability of medium- and high-copy-number versions caters to diverse experimental needs in molecular biology research.

Related Experiment Videos