Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chronic staphylococcal osteomyelitis: a new experimental rat model

N Spagnolo1, F Greco, A Rossi

  • 1Department of Microbiology, Catholic University of the Sacred Heart, Rome, Italy.

Infection and Immunity
|December 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitomycin C and vindesine: an ineffective combination chemotherapy in the treatment of malignant pleural mesothelioma.

Tumori·1992
Same author

Neoadjuvant chemotherapy with cisplatin, epirubicin and VP-16 for stage IIIA-IIIB non-small-cell lung cancer: a pilot study.

Tumori·1992
Same author

Supraspinal influences on recurrent inhibition in humans. Paralysis of descending control of Renshaw cells in patients with mental retardation.

Electroencephalography and clinical neurophysiology·1992
Same author

Expression in murine and human neuroblastoma cell lines of VGF, a tissue specific protein.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·1992
Same author

Calcium entry blockade as a mechanism for chlordimeform-induced inhibition of motor activity in the isolated guinea-pig ileum.

Pharmacology & toxicology·1992
Same author

Energy metabolism of the hypertrophied heart studied by 31P nuclear magnetic resonance.

Cardioscience·1992

This study developed a reliable rat model for chronic staphylococcal osteomyelitis using fibrin glue and Staphylococcus aureus. The model effectively mimics human post-traumatic osteomyelitis, aiding future research.

Area of Science:

  • Biomedical Science
  • Infectious Disease Research
  • Skeletal Biology

Background:

  • Chronic osteomyelitis is a challenging bone infection.
  • Existing animal models often use sclerosing agents or foreign bodies, which can confound results.
  • A need exists for a more physiologically relevant model of post-traumatic osteomyelitis.

Purpose of the Study:

  • To develop and validate a novel rat model for chronic staphylococcal osteomyelitis.
  • To utilize fibrin glue as a biocompatible matrix for bacterial inoculation.
  • To assess the reproducibility and characteristics of induced osteomyelitis lesions.

Main Methods:

  • Induction of osteomyelitis in rat tibias using fibrin glue and Staphylococcus aureus.
  • Evaluation of lesions through radiographic, macroscopic, and histological examinations.

Related Experiment Videos

  • Quantification of bacterial load via bone cultures at various time points (1-6 months).

    • Main Results:

    • Over 90% of inoculated tibias developed radiographic and histological evidence of osteomyelitis.
    • More than 80% exhibited gross pathology including bone deformation and abscesses.
    • Bacterial cultures were positive in 86% of cases, with control groups showing no lesions.

    Conclusions:

    • The fibrin glue-based rat model reliably reproduces chronic staphylococcal osteomyelitis.
    • This model closely mimics human post-traumatic osteomyelitis.
    • It is suitable for pathophysiological and therapeutic investigations.