Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Use of the E test for methicillin sensitivity testing

G E Bignardi1, U Riley, C S Easmon

  • 1Microbiology Department, St Mary's Hospital, London, UK.

The Journal of Antimicrobial Chemotherapy
|July 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeted Antibiotic Prophylaxis for Transrectal Fiducial Marker Insertion for Prostate Radiotherapy.

Clinical oncology (Royal College of Radiologists (Great Britain))·2015
Same author

Increase in reported Clostridium difficile cases as unintended consequence of enhanced norovirus testing.

The Journal of hospital infection·2013
Same author

Two-stage algorithm for Clostridium difficile: glutamate-dehydrogenase-positive toxin-negative enzyme immunoassay results may require further testing.

The Journal of hospital infection·2013
Same author

The efficacy of preventing neutropenic sepsis in patients with testicular germ cell tumours: results of two consecutive audits.

Clinical oncology (Royal College of Radiologists (Great Britain))·2010
Same author

MRSA decolonization with Prontoderm compared with chlorhexidine and mupirocin.

The Journal of hospital infection·2010
Same author

Glutamate dehydrogenase as confirmatory test for Clostridium difficile toxin A/B-positive stools.

The Journal of hospital infection·2010

The E test accurately detects methicillin resistance in staphylococci when using Mueller-Hinton agar supplemented with 5% NaCl. Optimization, including heavy inocula and a 4 mg/L breakpoint, ensures reliable methicillin-susceptibility testing.

Area of Science:

  • Clinical Microbiology
  • Antimicrobial Susceptibility Testing

Background:

  • Accurate detection of methicillin resistance in Staphylococcus is crucial for effective treatment.
  • Previous methods, like strip diffusion, have yielded equivocal results for some strains.

Purpose of the Study:

  • To evaluate the performance of the E test for methicillin sensitivity testing in Staphylococcus.
  • To identify optimal conditions for E test accuracy in detecting methicillin resistance.

Main Methods:

  • The E test was performed on 21 methicillin-resistant and 28 methicillin-sensitive Staphylococcus strains.
  • Testing involved Mueller-Hinton agar incubated at 30°C for 24 hours, with and without 5% NaCl supplementation.
  • Inoculum density and a breakpoint of 4 mg/L were investigated.

Related Experiment Videos

Main Results:

  • The E test demonstrated good performance with Mueller-Hinton agar supplemented with 5% NaCl.
  • Testing on agar without salt was essential for identifying methicillin resistance in one specific strain.
  • A breakpoint of 4 mg/L and heavy inocula are recommended for optimal results.

Conclusions:

  • The E test, with appropriate modifications (5% NaCl supplementation, heavy inocula, 4 mg/L breakpoint), is a reliable method for methicillin sensitivity testing in Staphylococcus.
  • Incorporating a control strain on the same plate aids in validating E test results.