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Method for computing the oxidation of two 13C-substrates ingested simultaneously during exercise

F Péronnet1, E Adopo, D Massicotte

  • 1Département d'éducation physique, Université de Montréal, P.Q., Canada.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|September 1, 1993
PubMed
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This study introduces a novel 13C labeling method to quantify simultaneous oxidation of glucose and fructose during exercise. The method accurately determined that 9.8g glucose and 5.7g fructose were oxidized in healthy males.

Area of Science:

  • Exercise Physiology
  • Nutritional Biochemistry
  • Metabolic Research

Background:

  • Determining substrate oxidation during exercise is crucial for understanding energy metabolism.
  • Previous methods often struggle to differentiate between simultaneously ingested carbohydrates.

Purpose of the Study:

  • To present and validate a new 13C labeling technique for quantifying the oxidation of two concurrently ingested exogenous substrates (glucose and fructose) during prolonged exercise.
  • To assess the oxidation rates of glucose and fructose when ingested together during moderate-intensity exercise.

Main Methods:

  • Developed a method utilizing 13C labeling to measure the total volume of 13CO2 produced (V13CO2tot).
  • The method solves the equation V13CO2tot = V13CO2endo + V13CO2A + V13CO2B using data from three experiments with varying isotopic compositions.

Related Experiment Videos

  • Applied the method to five healthy males exercising on a cycle ergometer at 65% maximal O2 uptake after ingesting a mixture of 15g glucose and 15g fructose.

    • Main Results:

    • The study successfully computed the oxidation amounts of simultaneously ingested glucose and fructose.
    • Results showed that 9.8 ± 3.1 g of glucose and 5.7 ± 2.1 g of fructose were oxidized.
    • The total exogenous carbohydrate oxidation (15.5 ± 4.3 g) aligns with previous findings for glucose oxidation alone.

    Conclusions:

    • The 13C labeling method is effective for quantifying the oxidation of multiple exogenous carbohydrate sources during exercise.
    • Glucose oxidation was higher than fructose oxidation when ingested in equal amounts during prolonged exercise.
    • Findings support existing literature on differential oxidation rates of glucose and fructose.