Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

HEPES buffer perfusate alters rabbit lung endothelial permeability

G C Douglas1, J A Swanson, D F Kern

  • 1Department of Physiology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City 37614-0576.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|September 1, 1993
PubMed
Summary

N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES) buffer can increase vascular permeability in perfused lungs, even without altering hemodynamics. Researchers recommend caution when using HEPES as a physiological buffer in organ systems.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thank you for not flowering: conservation genetics and gene flow analysis of native and non-native populations of Fraxinus (Oleaceae) in Ireland.

Heredity·2014
Same author

Self fertilization and seed set in Trifolium repens L. by in situ and in vitro pollination.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Pollination in vitro: effects on the growth of pollen tubes, seed set and gametophytic self-incompatibility in Trifolium pratense L. and T. repens L.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Methods for non-stigmatic pollination in Trifolium repens (Papilionaceae): seed set with self- and cross-pollinations in vitro.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Formation of Adventitious Buds in Stem Internodes of Populus spp. Cultured in vitro on Basal Medium: Influence of Endogenous Properties of Explants.

Journal of plant physiology·2012
Same author

Manipulation of shoot formation in cultured explants.

Methods in molecular biology (Clifton, N.J.)·2011

Area of Science:

  • Physiology
  • Biochemistry
  • Cell Biology

Background:

  • N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) is a commonly used zwitterionic buffer in biological research.
  • Previous studies indicated HEPES can alter endothelial cells and smooth muscle function at specific concentrations.

Purpose of the Study:

  • To investigate the impact of HEPES buffer on vascular permeability in isolated perfused rabbit lungs.
  • To compare the effects of HEPES with a traditional phosphate buffer on endothelial function.

Main Methods:

  • Isolated perfused rabbit lungs were used to measure hemodynamic parameters and protein permeability-surface area products (PS).
  • Endothelial permeability was assessed using both anionic and cationic albumin to evaluate charge effects.
  • Perfusion buffers included HEPES and phosphate at varying concentrations.

Related Experiment Videos

Main Results:

  • HEPES did not alter vascular pressure or resistance, but significantly increased endothelial permeability.
  • Both anionic and cationic albumin permeability increased with 12 mM HEPES compared to phosphate buffer.
  • While cationic albumin permeability returned to baseline at 60 mM HEPES, anionic albumin permeability remained elevated.

Conclusions:

  • HEPES buffer is not physiologically inert and can increase vascular permeability in organ systems.
  • The findings suggest that HEPES should be used with caution in experiments involving perfused organs.
  • Endothelial barrier function may be compromised by HEPES, necessitating careful buffer selection.