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Related Experiment Videos

Two overlapping genes encoding membrane proteins required for bacteriophage N4 adsorption

D R Kiino1, M S Singer, L B Rothman-Denes

  • 1Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.

Journal of Bacteriology
|November 1, 1993
PubMed
Summary

Researchers identified two genes, nfrA and nfrB, essential for bacteriophage N4 entry into host cells. The nfrA gene codes for an outer membrane protein acting as the receptor, while nfrB encodes an inner membrane protein possibly involved in the adsorption process.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Bacteriophage N4 adsorption is a critical step in its infection cycle.
  • The specific molecular mechanisms and genetic determinants of N4 adsorption are not fully elucidated.

Purpose of the Study:

  • To identify and characterize the genes responsible for bacteriophage N4 adsorption.
  • To elucidate the roles of these genes in the phage-host interaction.

Main Methods:

  • Nucleotide sequencing of genes involved in bacteriophage N4 adsorption.
  • Analysis of gene products' cellular localization and potential function.

Main Results:

  • The nucleotide sequences of two genes, nfrA and nfrB, were determined.

Related Experiment Videos

  • The nfrA gene encodes a 122-kDa outer membrane protein, identified as the putative phage receptor.
  • The nfrB gene encodes an 85-kDa inner membrane protein, potentially a component of the receptor complex.

    • Conclusions:

    • Two novel genes, nfrA and nfrB, are essential for bacteriophage N4 adsorption.
    • The nfrA gene product is the primary receptor for bacteriophage N4 on the host cell surface.
    • The nfrB gene product may play a supporting role in the phage receptor complex.