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Use of Salmonella phage P22 for transduction in Escherichia coli
B L Neal1, P K Brown, P R Reeves
1Department of Microbiology, University of Sydney, New South Wales, Australia.
Journal of Bacteriology
|November 1, 1993
Abstract:
A cosmid (pPR1347) carrying both the rfb gene cluster and the rfc gene of a Salmonella group B serovar has been constructed; Escherichia coli K-12 strains carrying this cosmid produce long-chain O antigen, are sensitive to phage P22, and can be transduced by P22. Some of the benefits of P22 transduction are now available for studying E. coli and potentially other genera.