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Related Experiment Videos

Analysis of a pause transfer sequence from apolipoprotein B

S L Chuck1, V R Lingappa

  • 1Department of Microbiology and Immunology, Physiology, University of California, San Francisco 94143-0444.

The Journal of Biological Chemistry
|October 25, 1993
PubMed
Summary

Apolipoprotein B uses pause transfer sequences for endoplasmic reticulum translocation. These sequences function independently of each other and protein synthesis, clarifying the mechanism of translocational pausing.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Typical secretory proteins translocate smoothly into the endoplasmic reticulum.
  • Apolipoprotein B exhibits unique pauses during translocation, mediated by specific sequences.
  • These pause transfer sequences can induce similar pausing in other proteins.

Purpose of the Study:

  • To investigate the independence of multiple pause transfer sequences in apolipoprotein B.
  • To identify key residues within the B' pause transfer sequence responsible for pausing.
  • To determine if translocational pausing is linked to translational pausing.

Main Methods:

  • Analysis of apolipoprotein B translocation dynamics.
  • Functional characterization of pause transfer sequences in chimeric proteins.

Related Experiment Videos

  • Site-directed mutagenesis to identify critical residues in the B' pause transfer sequence.

    • Main Results:

    • Pause transfer sequences act independently and are not dependent on upstream sequences.
    • Translocational pausing occurs independently of ongoing protein synthesis (translation).
    • The B' pause transfer sequence has specific structural features essential for its function.

    Conclusions:

    • Pause transfer sequences are independent functional units.
    • Translocational pausing is distinct from translational pausing.
    • Characterization of pause transfer sequences aids in identifying similar elements in other proteins and elucidates the mechanism of translocational pausing.