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High density lipoprotein conversion mediated by human plasma phospholipid transfer protein
A Y Tu1, H I Nishida, T Nishida
1Burnsides Research Laboratory, Department of Food Science, University of Illinois, Urbana 61801.
The Journal of Biological Chemistry
|November 5, 1993
Summary
Phospholipid transfer protein (PLTP) purifies from human plasma and exhibits both phospholipid transfer and high-density lipoprotein (HDL) conversion activity. This protein plays a key role in HDL enlargement, with its activity inhibited by specific antibodies and free fatty acids.
Area of Science:
- Biochemistry
- Lipid Metabolism
- Protein Purification
Background:
- Phospholipid transfer protein (PLTP) is a key enzyme in lipoprotein metabolism.
- Understanding PLTP's function is crucial for comprehending high-density lipoprotein (HDL) remodeling.
Purpose of the Study:
- To purify and characterize phospholipid transfer protein (PLTP) from human plasma.
- To investigate the role of PLTP in high-density lipoprotein (HDL) conversion and enlargement.
Main Methods:
- Purification of PLTP using sequential column chromatography.
- Analysis of purified PLTP by SDS-PAGE and isoelectric focusing.
- Assay of PLTP's phospholipid transfer and HDL conversion activities.
- Inhibition studies using anti-PLTP immunoglobulin G and free fatty acids.
Main Results:
- Purified PLTP (78 kDa) demonstrated microheterogeneity.
- PLTP exhibited both phospholipid transfer and HDL conversion activities, enlarging HDL3 to HDL2b.
- Anti-PLTP immunoglobulin G effectively inhibited PLTP's activities.
- HDL enlargement involved apoprotein release, primarily apoA-I.
- Free fatty acids inhibited PLTP-mediated HDL enlargement.
Conclusions:
- PLTP is a significant factor in human plasma HDL enlargement.
- PLTP's enzymatic activities are essential for HDL remodeling.
- Inhibition of PLTP offers a potential therapeutic target for modulating HDL levels.