Related Experiment Videos
Identification of the nerve terminal targets of botulinum neurotoxin serotypes A, D, and E
G Schiavo1, O Rossetto, S Catsicas
1Centro Consiglio Nazionale delle Ricerche Biomembrane, Università di Padova, Italy.
The Journal of Biological Chemistry
|November 15, 1993
Abstract:
Botulinum neurotoxins are metalloproteins with one zinc atom bound to the zinc binding motif of zinc endopeptidases. Here we show that botulinum neurotoxin serotypes A, D, and E are zinc endoproteases specific for components of the synaptic vesicle docking and fusion complex. Serotypes A and E cleave SNAP-25, a 25-kDa protein of the synaptic terminal, while serotype D is specific for VAMP/synaptobrevin, a membrane protein of synaptic vesicles. Both rat brain VAMP isoforms are cleaved at a single Lys-Leu peptide bond. The proteolytic activity of these neurotoxins is inhibited by EDTA and captopril.