Related Experiment Videos
Determination of immune complexes by high-performance gel chromatography (positive cooperativity of antibody-antigen
1Institute of Applied Microbiology, School of Food and Biotechnology, University of Bodenkultur, Vienna, Austria.
Journal of Biochemical and Biophysical Methods
|September 1, 1993
Abstract:
Positive cooperativity of antibody-antigen complex formation using human Cu/Zn superoxide dismutase and a murine monoclonal antibody as model is demonstrated by high-performance gel chromatography using TSK G3000sw and TSK G4000sw columns. Three different antibody-antigen complexes named Type I, II and III composed from two antigen molecules+two antibody molecules, two antigen molecules+one antibody molecule and one antigen molecule+one antibody molecule could be separated. The degree and ratio of complexes present in solution depends mainly on the ratios of antibody and antigen.