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Reduced binding capacity of concanavalin A-sepharose after treatment with chaotropic agents
M Fountoulakis1, J F Juranville
1F. Hoffmann-La Roche Ltd., Pharmaceutical Research-New Technologies, Department of Biology, Basel, Switzerland.
Journal of Biochemical and Biophysical Methods
|September 1, 1993
Abstract:
Affinity chromatography on Concanavalin A-Sepharose is widely used for purification of glycoproteins carrying mannose and glucose oligosaccharide moieties. High concentrations of chaotropes elute Concanavalin A from the gel matrix and significantly reduce the binding capacity of the column.