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Administration of inactivated and detergent-treated influenza virus to mice before virus challenge reduces mortality
1Institut Armand Frappier, Université du Québec, Laval des Rapides, Canada.
Abstract:
Formalin-inactivated virus (FIV) and the detergent-treated virus (DTV) preparations were tested for their ability to enhance the resistance of mice to experimental influenza infection. FIV (100 micrograms) was administered intravenously to mice. After 24 hr, animals were challenged with 5 LD50 dose intranasally. FIV-treated and non-treated (control) mice had 10% and 100% mortality, respectively. Similar results were obtained with the DTV (40 micrograms) preparation. The pulmonary virus titer of FIV-treated mice was lower when compared with the control. Mechanisms other than acquired immunity may have conferred the early resistance to virus infection in mice.
Insights
Formalin-inactivated virus (FIV) and detergent-treated virus (DTV) protect mice against influenza. These viral preparations significantly reduced mortality and viral load in mice, suggesting non-immune mechanisms may be involved.
Area of Science:
- Virology
- Immunology
- Infectious Diseases
Background:
- Influenza remains a significant global health threat, necessitating novel prophylactic strategies.
- Understanding early resistance mechanisms is crucial for developing effective antiviral interventions.
Purpose of the Study:
- To evaluate the efficacy of Formalin-inactivated virus (FIV) and detergent-treated virus (DTV) in enhancing resistance to experimental influenza infection in mice.
- To investigate the impact of these viral preparations on mortality and viral load.
Main Methods:
- Mice were intravenously administered with either FIV (100 µg) or DTV (40 µg).
- Following a 24-hour incubation period, mice were intranasally challenged with a lethal dose (5 LD50) of influenza virus.
- Mortality rates and pulmonary virus titers were assessed in treated and control groups.
Main Results:
- FIV treatment resulted in a significant reduction in mortality (10%) compared to control mice (100%).
- DTV treatment showed similar protective effects, with reduced mortality.
- Pulmonary virus titers were notably lower in FIV-treated mice compared to controls.
Conclusions:
- Both FIV and DTV preparations demonstrate potent protective effects against experimental influenza infection in mice.
- The observed early resistance may involve mechanisms beyond traditional acquired immunity.
- These findings highlight the potential of inactivated and treated virus preparations as prophylactic agents against influenza.