Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antenatal sex determination in Macaca arctoides

M Daniels, H C Hughes, M Lang

    Fertility and Sterility
    |September 1, 1976
    PubMed
    Summary

    Accurate prenatal sex determination in Macaca arctoides was achieved using amniotic cell analysis. The Papanicolaou stain method reliably differentiated male and female fetuses based on X-chromatin (Barr body) presence.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Secondary solution for breast reconstruction following total DIEP flap loss: A single-center experience after 3270 DIEP flaps.

    Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2024
    Same author

    G-FORCE: the effectiveness of group psychotherapy for Cluster-C personality disorders: protocol of a pragmatic RCT comparing psychodynamic and two forms of schema group therapy.

    Trials·2023
    Same author

    The BUrn Mortality Prediction (BUMP) Score - An improved mortality prediction score based on data of the German burn registry.

    Burns : journal of the International Society for Burn Injuries·2022
    Same author

    Influence Of Pre-Existing Neurological Illnesses On The Outcome Of Severe Burn Injuries.

    Annals of burns and fire disasters·2021
    Same author

    Human pituitary adenomas do not overexpress thec-erbB-2 oncoprotein.

    Endocrine pathology·2020
    Same author

    Expanded analysis of secondary germline findings from matched tumor/normal sequencing identifies additional clinically significant mutations.

    JCO precision oncology·2019

    Area of Science:

    • Primate reproductive biology
    • Fetal genetics
    • Cellular morphology

    Background:

    • Antenatal sex determination is crucial for reproductive studies in non-human primates.
    • Accurate methods are needed for assessing fetal development and genetic makeup in Macaca arctoides.

    Purpose of the Study:

    • To evaluate the efficacy of transabdominal amniocentesis for fetal sex determination in Macaca arctoides.
    • To compare Y-chromatin fluorescence and X-chromatin (Barr body) analysis for accuracy.

    Main Methods:

    • Transabdominal amniocentesis was performed on 11 Macaca arctoides between 10-20 weeks of gestation.
    • Amniotic cells were analyzed using Y-chromatin fluorescence and a modified Papanicolaou staining technique.

    Main Results:

    Keywords:
    AmniocentesisAnimals, LaboratoryClinical ResearchExaminations And DiagnosesGenetic TechnicsLaboratory Examinations And DiagnosesResearch MethodologySex Determination

    Related Experiment Videos

    • Y-chromatin fluorescence failed to accurately predict fetal sex.
    • Papanicolaou staining allowed for complete and accurate sex differentiation.
    • Male fetuses showed <10% X-chromatin (Barr bodies); female fetuses showed >45% X-chromatin.

    Conclusions:

    • Modified Papanicolaou staining of amniotic cells is a reliable method for antenatal sex determination in Macaca arctoides.
    • X-chromatin analysis is superior to Y-chromatin fluorescence for this purpose in this species.