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Correlation between electroconvulsive seizure and HSC70 mRNA induction in mice brain
1Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Neuroscience Letters
|July 23, 1993
Summary
Electroconvulsive seizures increase heat shock cognate protein (HSC70) mRNA in mouse brains. Drug effects varied, with diazepam attenuating induction and phenytoin delaying it, suggesting HSC70 mRNA levels do not directly correlate with seizure severity.
Area of Science:
- Neuroscience
- Molecular Biology
- Pharmacology
Background:
- Heat shock proteins (HSPs) play crucial roles in cellular stress responses.
- Understanding HSP induction by electroconvulsive seizures (ECS) is vital for neurological research.
- The differential effects of anticonvulsant drugs on HSP induction require further investigation.
Purpose of the Study:
- To investigate the impact of electroconvulsive seizures (ECS) on heat shock protein (HSP) mRNA induction in the mouse brain.
- To examine the modulatory effects of diazepam and phenytoin on ECS-induced HSP mRNA expression.
Main Methods:
- Mice were subjected to electroconvulsive seizures (ECS).
- mRNA levels of heat shock cognate protein (HSC70) and heat shock protein (HSP70) were analyzed post-ECS.
- The influence of diazepam and phenytoin on ECS-induced mRNA changes was evaluated.
Main Results:
- ECS significantly induced heat shock cognate protein (HSC70) mRNA, but not heat shock protein (HSP70) mRNA.
- HSC70 mRNA induction peaked at 1 hour post-ECS and persisted for several hours.
- Diazepam attenuated HSC70 mRNA induction, while phenytoin delayed it without reducing the peak level.
Conclusions:
- Electroconvulsive seizures robustly induce HSC70 mRNA in the mouse brain.
- The level of HSC70 mRNA induction does not necessarily correlate with seizure severity.
- Anticonvulsant drugs differentially modulate the ECS-induced HSC70 mRNA response.