Fractionation of RNA from tetahymena by affinity chromatography on poly-U-Sepharose

Exponential growing Tetrahymena pyriformis organisms were labelled with (3H) uridine or (3H) adenosine. The labelled RNA was extracted and isolated by affinity chromatography on poly-uridylic-acid sepharose and further analysed by means of sucrose gradient centrifugation and RNase digestion. Experimental evidence proved the existence of RNase resistant poly adenylic-acid fragments in the RNA of Tetrahymena cells. This poly adenylic-acid segment has a sedimentation rate of 4-5 S and would be localised in the 10-12S region of the RNA which is probably the m-RNA.