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Crustacean cardioactive peptide in the sphinx moth, Manduca sexta
H K Lehman1, C M Murgiuc, T A Miller
1ARL Division of Neurobiology, University of Arizona, Tucson 85721.
Peptides
|July 1, 1993
Summary
Crustacean cardioactive peptide (CCAP) was found in the sphinx moth Manduca sexta, acting as a neurotransmitter. This peptide influences muscle contractions in insects, similar to its role in crustaceans.
Area of Science:
- Neurobiology
- Insect Physiology
- Peptide Chemistry
Background:
- Crustacean cardioactive peptide (CCAP) is a known neuropeptide involved in muscle regulation in crustaceans.
- The presence and function of CCAP in insects have not been extensively studied.
Purpose of the Study:
- To isolate and identify CCAP in the sphinx moth Manduca sexta.
- To determine the physiological actions of CCAP on insect muscles.
Main Methods:
- Quantification of CCAP-like immunoreactivity using ELISA.
- Purification and sequencing of the peptide via amino acid analysis, mass spectrometry, and HPLC.
- Testing the effects of CCAP on isolated insect heart and leg muscles.
Main Results:
- CCAP was identified in the abdominal CNS of Manduca sexta with the sequence Pro-Phe-Cys-Asn-Ala-Phe-Thr-Gly-Cys-NH2.
- CCAP demonstrated excitatory actions on the Manduca sexta heart and the Schistocerca americana extensor-tibia muscle.
- Purified and synthetic CCAP produced identical effects, confirming its identity and activity.
Conclusions:
- CCAP is present in the insect Manduca sexta.
- CCAP exerts potent neurotransmitter or neurohormonal effects on various insect muscles, indicating conserved function across arthropods.