[Detergents and precipitation of serum lipoproteins by antisera (author's transl)]

By using gel diffusion methods, it was shown that during immunoelectrophoresis and double diffusion in two dimensions, anionic detergents (sodium oleate, sodium desoxycholate, sodium lauryl-sulfate and sodium decyl-sulfate), added to human serum at the final concentration of 0.5%, do not prevent the formation of an insoluble antigen-antibody complex (precipitation line) when specific antibodies react with serum lipoproteins: LDL, HDL and VLDL. In spite of the presence of anionic detergents, the antigen-antibody interaction in gel occurs even if the amount of lipoproteins in serum was highly reduced. On the contrary, the non ionic detergent tween 80, has a strong inhibitory effect on the specific precipitation of lipoproteins, especially in the case of HDL.