[Effects of epidermal growth factor on growth and differentiation of rat granulosa cells]

It was known that epidermal growth factor (EGF) plays an important role in the regulation of reproduction. The present study was undertaken to investigate the effect of EGF on the proliferation and differentiation of cultured rat granulosa cells. The results showed that EGF inhibited the 3H-TdR incorporation into DNA of granulosa cells, while the progesterone production was increased due to enhanced 3 beta-HSD activity. Radioreceptor assay (RRA) suggested that there were specific receptors for EGF on the granulosa cell with a Kd of 1.83 +/- 0.30 x 10(-8) mol/L and a Bmax of 1.75 +/- 0.29 x 10(4) sites/cell. Using method of immunohistochemistry, no EGF-like immunoreactivity was found in the granulosa cells at different age or different estrous cycle, but in the theca folliculi, interstitium and corpus luteum. These results suggest that EGF can regulate the growth and differentiation of the granulosa cells in the course of maturation of the folliculi and granulosa cells.