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Updated: Aug 9, 2026
Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
Published on: June 5, 2015
[In vitro granulopoietic cultures. Analysis using monoclonal antibodies]
F Puente1, F Gómez Casal, C Vázquez
1Servicio de Hematología, Hospital Clínico Universitario, Zaragoza.
Abstract:
Four monoclonal antibodies were applied, using an immunoenzymatic technique, the alkaline phosphatase anti-alkaline phosphatase, in granulopoietic cultures in vitro. In this manner, granulopoietic growth at tenth day of culture has been defined as positive for myeloid markers (CD15, CD11b) and negative for macrophagic (CD68) and proliferating cells (Ki 67) markers.
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