In situ hybridizations of Eperythrozoon suis visualized by electron microscopy
Related Experiment Video
Updated: Nov 8, 2026

Visualization of the Superior Ocular Sulcus during Danio rerio Embryogenesis
Published on: March 27, 2019
S M Gwaltney1, L H Willard, R D Oberst
1Department of Pathology and Microbiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506.
Abstract:
Eperythrozoon suis is an extracellular red blood cell parasite that causes icteroanemia and poor growth performance in feeder pigs and has been associated with anemias in baby pigs and reproductive failures in sows. At present, few efficient tests are available for the diagnosis and study of E. suis infection in swine. This report discusses how a recently developed recombinant DNA probe (KSU-2) specific to E. suis DNA was utilized in in situ DNA hybridizations that couple biotinylated TaqI digested products of KSU-2 DNA with an immunogold detection system allowing the visualization by transmission electron microscopy of the gold particles within eperythrozoon organisms. Specific labelling by the probe of eperythrozoon organisms demonstrated a pattern that changed with the various stages of the eperythrozoon life cycle.
More Related Videos
09:33Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
Published on: November 18, 2017
07:36In Situ Hybridization Combined with Immunohistochemistry in Cryosectioned Zebrafish Embryos
Published on: March 3, 2022