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Mitogenic effect of lymph, amniotic and allantoic fluid in bovine fetal myoblasts
A Orzechowski1, S Blachowski, T Motyl
1Department of Animal Physiology, Veterinary Faculty, Warsaw Agricultural University, Poland.
Zentralblatt Fur Veterinarmedizin. Reihe A
|August 1, 1993
Abstract:
Amniotic and allantoic fluids, as well as afferent lymph (tissue fluid), stimulated DNA synthesis in bovine fetal myoblasts (BFM) at low concentrations (5%). Higher doses abolished the mitostimulatory effect of these fluids and led toward inhibition of [3H]mTdR incorporation into DNA. These effects were much more evident (particularly in the case of amniotic fluid) when fluids were lyophylized prior to the experiment. BFM protein synthesis was accelerated by lymph in a dose dependent manner.