Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

External Ca effect on water permeability, regulatory volume decrease, and extracellular space in barnacle muscle

D M Berman1, C Peña-Rasgado, M Holmgren

  • 1Department of Physiology and Biophysics, University of Health Sciences Chicago Medical School, Illinois 60064.

The American Journal of Physiology
|October 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Proteomic profiling identifies muscle-invasive bladder cancers with distinct biology and responses to platinum-based chemotherapy.

Nature communications·2025
Same author

Proteomic profiling identifies muscle-invasive bladder cancers with distinct biology and responses to platinum-based chemotherapy.

Nature communications·2025
Same author

Multimodal Biomarkers That Predict the Presence of Gleason Pattern 4: Potential Impact for Active Surveillance.

The Journal of urology·2023
Same author

Microencapsulated islet allografts in diabetic NOD mice and nonhuman primates.

European review for medical and pharmacological sciences·2020
Same author

Author Correction: Femtosecond Laser Mass Spectrometry and High Harmonic Spectroscopy of Xylene Isomers.

Scientific reports·2018
Same author

Femtosecond Laser Mass Spectrometry and High Harmonic Spectroscopy of Xylene Isomers.

Scientific reports·2018
Same journal

Blood coagulation in fish.

The American journal of physiology·2011
Same journal

Renal tubular reabsorption, metabolic utilization and isomeric fractionation of lactic acid in the dog.

The American journal of physiology·2010
Same journal

The inactivation of placental toxin by human serum.

The American journal of physiology·2010
Same journal

Adrenal function following ovariectomy in the rat.

The American journal of physiology·2010
Same journal

Capillary permeability; perfusion of frog and guinea pig hind limbs.

The American journal of physiology·2010
Same journal

Evaluation of protective measures against sunburn.

The American journal of physiology·2010
See all related articles

Extracellular calcium (Cao) does not affect water permeability but is crucial for regulatory volume decrease (RVD) in barnacle muscle cells. Cells require Cao to initiate RVD in hypotonic solutions, demonstrating a calcium-dependent response.

Area of Science:

  • Cell Physiology
  • Biophysics
  • Marine Biology

Background:

  • Sarcolemmal hydraulic water permeability (L'p) is vital for cell volume regulation.
  • Regulatory volume decrease (RVD) is a cellular response to osmotic stress.
  • Extracellular space (ECS) dynamics influence cellular transport and function.

Purpose of the Study:

  • To investigate the role of extracellular calcium (Cao) in barnacle muscle cell L'p, RVD, and ECS.
  • To determine the concentration-dependent effect of Cao on RVD.
  • To analyze the impact of Cao and hypotonicity on apparent ECS.

Main Methods:

  • Measurement of L'p using osmotic gradients.
  • Induction of RVD under varying Cao concentrations and hypotonic conditions.

Related Experiment Videos

  • Analysis of ECS using three-dimensional autoradiographic reconstruction.

    • Main Results:

    • Cao absence or presence did not alter L'p.
    • Cells behaved as osmometers in the short term, irrespective of Cao.
    • Cao significantly influenced RVD magnitude and initiation in hypotonic solutions, with half-maximal effect at 4.83 mM Cao.
    • Both Cao and hypotonicity reduced apparent ECS.

    Conclusions:

    • Extracellular calcium is essential for initiating RVD in barnacle muscle cells under hypotonic stress.
    • L'p and short-term osmotic behavior are independent of Cao.
    • Cao and hypotonicity modulate ECS, suggesting complex cellular volume regulation mechanisms.