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Kallikrein release by vascular tissue

H Nolly1, O A Carretero, A G Scicli

  • 1Hypertension and Vascular Research Division, Henry Ford Hospital, Detroit, Michigan 48202.

The American Journal of Physiology
|October 1, 1993
PubMed
Summary

Vascular tissue releases kallikrein (a key enzyme in the kallikrein-kinin system), with higher concentrations in smaller vessels. Protein synthesis inhibition blocks this release, indicating de novo synthesis.

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Area of Science:

  • Biochemistry
  • Physiology
  • Vascular Biology

Background:

  • Vascular tissue contains kallikrein and its mRNA, suggesting an intrinsic vascular kallikrein-kinin system.
  • The distribution and release mechanisms of vascular kallikrein remain largely uncharacterized.

Purpose of the Study:

  • To investigate variations in kallikrein concentration across different vascular beds.
  • To determine if vascular tissue actively releases kallikrein.
  • To ascertain if de novo synthesis is involved in kallikrein release.

Main Methods:

  • Rat vascular rings and isolated-perfused hindquarters were utilized to measure kallikrein and kininogenase activity.
  • Kallikrein release was quantified in incubation baths and perfusates over time.
  • The effect of puromycin, a protein synthesis inhibitor, on kallikrein release was assessed.

Main Results:

  • Kallikrein concentration was significantly higher in smaller arteries and veins (tail arteries/veins) compared to larger vessels (aorta/vena cava).
  • Vascular rings demonstrated a continuous release of active and total kallikrein over a 3-hour period.
  • Puromycin pretreatment markedly reduced the release of total kallikrein into the perfusate, indicating synthesis-dependent release.

Conclusions:

  • Vascular tissue possesses a functional kallikrein-kinin system with distinct regional variations.
  • Vascular kallikrein is actively released by tissues, and this release is dependent on ongoing protein synthesis.
  • These findings suggest a localized, de novo synthesis and release mechanism for kallikrein within the vasculature.

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