Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Radioligand binding methods: practical guide and tips

D B Bylund¹, M L Toews

¹Department of Pharmacology, University of Nebraska Medical Center, Omaha 68198-6260.

The American Journal of Physiology

|November 1, 1993

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Changes in postnatal norepinephrine alter alpha-2 adrenergic receptor development.

Neuroscience·2011

Same author

Quantitative determination of lysophosphatidic acids (LPAs) in human saliva and gingival crevicular fluid (GCF) by LC-MS/MS.

Journal of pharmaceutical and biomedical analysis·2011

Same author

Lack of effect of the alpha2C-adrenoceptor Del322-325 polymorphism on inhibition of cyclic AMP production in HEK293 cells.

British journal of pharmacology·2010

Same author

Differential effects of neonatal norepinephrine lesions on immediate early gene expression in developing and adult rat brain.

Neuroscience·2008

Same author

Possible involvement of alpha-2A adrenergic receptors in attention deficit hyperactivity disorder: radioligand binding and polymorphism studies.

American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics·2006

Same author

Development of the norepinephrine transporter in the rat CNS.

Neuroscience·2004

Same journal

Blood coagulation in fish.

The American journal of physiology·2011

Same journal

Renal tubular reabsorption, metabolic utilization and isomeric fractionation of lactic acid in the dog.

The American journal of physiology·2010

Same journal

The inactivation of placental toxin by human serum.

The American journal of physiology·2010

Same journal

Adrenal function following ovariectomy in the rat.

The American journal of physiology·2010

Same journal

Capillary permeability; perfusion of frog and guinea pig hind limbs.

The American journal of physiology·2010

Same journal

Evaluation of protective measures against sunburn.

The American journal of physiology·2010

See all related articles

Radioligand binding assays are essential for studying receptor interactions and changes. This guide provides practical tips for optimizing these powerful assays and avoiding common pitfalls in receptor research.

Area of Science:

Pharmacology
Physiology
Biochemistry
Immunology
Cell Biology

Background:

Radioligand binding assays are versatile tools for investigating receptor-ligand interactions.
These assays are crucial for understanding the roles of hormones, neurotransmitters, and drugs.
They are widely employed across various scientific disciplines.

Purpose of the Study:

To provide a comprehensive overview of radioligand binding assay techniques.
To offer practical guidelines for researchers with limited experience in assay development.
To highlight common challenges and solutions in receptor binding studies.

Main Methods:

Detailed explanation of receptor preparation and radioligand selection.

Related Experiment Videos

Optimization of assay conditions and data analysis methodologies.

Comparison of membrane preparations versus intact cell binding assays.

Main Results:

Guidelines for setting up new radioligand binding assays.
Strategies for avoiding common experimental pitfalls.
Discussion of advantages and disadvantages of different assay formats.

Conclusions:

Radioligand binding assays are powerful tools for receptor characterization.
Proper assay design and execution are critical for reliable results.
Intact cell assays offer unique insights but require careful consideration of receptor regulation.