Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A quest for oleandrin in decayed human tissue

G Rule1, L G McLaughlin, J Henion

  • 1Diagnostic Laboratory, College of Veterinary Medicine, Cornell University, Ithaca, NY 14851-0786.

Analytical Chemistry
|October 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunoaffinity Ultrafiltration with Ion Spray HPLC/MS for Screening Small-Molecule Libraries.

Analytical chemistry·2011
Same author

Acceptance Criteria for Ultratrace HPLC-Tandem Mass Spectrometry:  Quantitative and Qualitative Determination of Sulfonylurea Herbicides in Soil.

Analytical chemistry·2011
Same author

Comparison between liquid chromatography-time of-flight mass spectrometry and selected reaction monitoring liquid chromatography-mass spectrometry for quantitative determination of idoxifene in human plasma.

Journal of chromatography. B, Biomedical sciences and applications·2001
Same author

A polymeric microfluidic chip for CE/MS determination of small molecules.

Analytical chemistry·2001
Same author

Chip-based quantitative capillary electrophoresis/mass spectrometry determination of drugs in human plasma.

Analytical chemistry·2001
Same author

A 384-well solid-phase extraction for LC/MS/MS determination of methotrexate and its 7-hydroxy metabolite in human urine and plasma.

Analytical chemistry·2001
Same journal

Heterojunction-Enhanced Interfacial Evanescent-Tunable Fiber Optic Probe for Amplification-free CRISPR/Cas12a-Based Rapid and Ultrasensitive Detection of MPXV.

Analytical chemistry·2026
Same journal

Tunable Charge Transfer in Europium Metal-Organic Frameworks for Ratiometric Sensing of a Sarin Simulant.

Analytical chemistry·2026
Same journal

A β-Cyclodextrin/Ag<sub>2</sub>O@MWCNT-Based Stochastic Platform for the Simultaneous Molecular Enantiorecognition and Enantioanalysis of Twelve Amino Acids in Biological Matrices.

Analytical chemistry·2026
Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026
Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026
Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026
See all related articles

Improved sample preparation techniques are crucial for analyzing decayed tissues. Coupling liquid chromatography with tandem mass spectrometry (LC/LC-MS/MS) offers effective cleanup for complex samples and enables high-throughput analysis.

Area of Science:

  • Forensic toxicology
  • Analytical chemistry

Background:

  • Analyzing trace analytes in decayed biological samples presents significant challenges due to matrix complexity.
  • Current literature lacks comprehensive information on modern sample preparation techniques for such samples.

Observation:

  • The study highlights the need for enhanced sample cleanup methods for severely decayed tissues.
  • Coupling two-dimensional liquid chromatography (LC/LC) with tandem mass spectrometry (MS/MS) proved effective for on-line sample cleanup.
  • This combined technique demonstrated improved selectivity for complex sample matrices.

Findings:

  • LC/LC-MS/MS enabled the semi-automated analysis of over 50 samples within three days.
  • The reliability and ruggedness of the LC/LC-MS/MS system suggest its suitability for large-scale biological sample analysis.

Related Experiment Videos

  • Immunoaffinity chromatography was proposed as a more selective method for trace enrichment of target analytes.

    • Implications:

    • The findings suggest that LC/LC-MS/MS is a powerful tool for forensic and clinical toxicology.
    • This approach could be valuable for routine analysis of biological samples like plasma and urine.
    • Further research into immunoaffinity-based sample preparation could enhance analytical sensitivity and selectivity.