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Ultrastructure of the developing stomach in human embryos
H Otani1, T Yoneyama, R Hashimoto
1Department of Anatomy, Shimane Medical University, Izumo, Japan.
Anatomy and Embryology
|February 1, 1993
Summary
This study details the ultrastructural development of the human embryonic stomach, identifying key timings for gastric pit formation, muscle layer development, and nerve fiber appearance. Carbohydrate presence in the gastric epithelium and serosa was also characterized during early gestation.
Area of Science:
- Developmental Biology
- Human Embryology
- Gastrointestinal Histology
Background:
- The early development of the human stomach is crucial for understanding gastrointestinal formation.
- Detailed ultrastructural analysis provides insights into the precise timing of organogenesis.
Purpose of the Study:
- To investigate the ultrastructural development of the human embryonic stomach.
- To precisely determine the timing of appearance for differentiated gastric structures.
- To analyze the distribution and changes in carbohydrates during stomach development.
Main Methods:
- Light, scanning electron, and transmission electron microscopy were used.
- Human embryos at Carnegie stages 14-23 (5-8 weeks gestation) were examined.
- Histocytochemical staining was employed to detect carbohydrates.
Main Results:
- Gastric pits first appeared at stage 22; myofibrils in the muscle layer were observed from stage 22.
- Nerve fibers were first seen at stage 16, forming a plexus later.
- Carbohydrates were consistently found in the gastric epithelium and, from stage 17, in the serosa.
Conclusions:
- The study provides a precise timeline for key ultrastructural events in human stomach development.
- Carbohydrate localization in the embryonic stomach epithelium and serosa was characterized.
- The findings contribute to understanding the complex interplay of developing gastric layers.