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Comparative study of the activity and composition of HDL3 in Russian and American men

Y A Shakhov1, J F Oram, N V Perova

  • 1Department of Medicine, University of Washington, Seattle 98195.

Arteriosclerosis and Thrombosis : a Journal of Vascular Biology
|December 1, 1993
PubMed

Insights

American high-density lipoprotein cholesterol (HDL) particles show greater protective function against heart disease than Russian HDL particles. This functional difference may explain higher coronary heart disease (CHD) risk in Russia.

Area of Science:

  • Cardiovascular Research
  • Lipid Metabolism
  • Population Health

Background:

  • Previous studies indicated a weaker inverse correlation between high-density lipoprotein cholesterol (HDL-C) and coronary heart disease (CHD) risk in Russian males compared to American males.
  • It was hypothesized that HDL particles might offer less protection against CHD in Russian populations.

Purpose of the Study:

  • To compare the functional activity and lipid composition of HDL3 particles from Russian and American men with varying HDL-C levels.
  • To investigate potential reasons for observed differences in CHD risk between populations.

Main Methods:

  • Isolation of HDL3 from blood plasma of men in Moscow and Seattle.
  • Assessment of HDL3 functional activity in stimulating cholesterol efflux from fibroblasts.
  • Measurement of HDL3's effect on cellular cholesterol esterification.
  • Analysis of HDL3 binding to cell-surface sites and apolipoprotein E content.

Main Results:

  • American HDL3 demonstrated higher activity in promoting cholesterol efflux and suppressing cellular cholesterol esterification compared to Russian HDL3, regardless of plasma HDL-C levels.
  • These functional differences persisted even after removing apolipoprotein E-containing particles.
  • While binding affinity to high-affinity sites was similar, Russian HDL3 exhibited higher non-specific binding.

Conclusions:

  • This study reveals functional disparities between HDL particles from Russian and American populations for the first time.
  • The reduced activity of Russian HDL3 in facilitating cholesterol efflux may contribute to the higher CHD risk observed in the Russian population.

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