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The RNA editing process in Trypanosoma brucei

K Stuart1

  • 1Seattle Biomedical Research Institute, WA 98109-1651.

Seminars in Cell Biology
|August 1, 1993
PubMed
Summary

RNA editing in Trypanosoma brucei involves adding or removing uridines in mitochondrial mRNAs. Guide RNAs (gRNAs) encoded by minicircles regulate this crucial process, varying across species and mutants.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Parasitology

Background:

  • Trypanosoma brucei exhibits extensive RNA editing in its mitochondrial mRNAs.
  • This editing process, involving uridine addition and deletion, is critical for producing functional transcripts.
  • The accumulation of edited RNAs is regulated throughout the parasite's life cycle.

Purpose of the Study:

  • To discuss the current understanding of RNA editing in Trypanosoma brucei.
  • To explore the roles of guide RNAs (gRNAs) and minicircle DNA in specifying editing.
  • To review the editing mechanism and machinery involved in post-transcriptional modification.

Main Methods:

  • Analysis of mitochondrial mRNA sequences and their edited forms.
  • Investigation of guide RNA (gRNA) encoding minicircles.
  • Comparative studies across different Trypanosoma brucei species and mutants.

Main Results:

  • Twelve mitochondrial mRNAs are subject to editing, with nine extensively modified by uridine insertion/deletion.
  • Hundreds of distinct gRNAs, encoded in minicircles, direct the specific editing events.
  • Minicircle DNA content explains variations in editing patterns observed between species and mutants.

Conclusions:

  • RNA editing is a complex, regulated process in Trypanosoma brucei mitochondria.
  • gRNAs and minicircle organization are key determinants of editing specificity and variation.
  • Further research is needed to fully elucidate the editing mechanism and machinery.

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