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Related Experiment Videos

Cellular relations in mouse circumvallate taste buds

R G Murray1

  • 1Medical Sciences Program, Indiana University Medical School, Bloomington 47405.

Microscopy Research and Technique
|October 15, 1993
PubMed
Summary

Electron microscopy revealed distinct differences in taste bud cell structures between mice and rabbits, particularly in Type I cells. No significant variations were found between the two mouse strains examined.

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Area of Science:

  • Cell biology
  • Sensory neuroscience
  • Histology

Background:

  • Mammalian taste buds are complex sensory organs responsible for gustation.
  • Circumvallate papillae house a high density of taste buds, crucial for taste perception.
  • Understanding taste bud ultrastructure aids in elucidating gustatory transduction mechanisms.

Purpose of the Study:

  • To investigate the fine structure of taste buds in circumvallate papillae of two mouse strains using electron microscopy.
  • To compare the ultrastructural morphology of mouse taste bud cells with those previously described in rabbits.
  • To document the cellular relationships and developmental stages within mammalian taste buds.

Main Methods:

  • Transmission electron microscopy was employed to examine taste bud fine structure.
  • Mice were anesthetized and perfused with a double aldehyde fixative.
  • Tissues were embedded in Epon, and semi-serial sections were analyzed.

Main Results:

  • Mouse taste bud morphology aligns with descriptions of mammalian taste buds innervated by the ninth cranial nerve.
  • Type II cells showed well-documented stages of origin, maturation, and degeneration.
  • Type I cells in mice possess large dense-cored granules, differing from rabbit Type I cells, and do not form a dense pore product.

Conclusions:

  • Significant ultrastructural differences exist in Type I taste bud cells between mice and rabbits.
  • The pore substance in mouse taste buds comprises small vesicles within a diffuse matrix, unlike the homogenous product in rabbits.
  • No significant ultrastructural differences were observed between the two mouse strains studied.

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