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Nimodipine prevents the in vivo decrease in hippocampal extracellular acetylcholine produced by hypobaric hypoxia

B Shukitt-Hale1, M J Stillman, A Levy

  • 1Military Performance and Neuroscience Division, United States Army Research Institute of Environmental Medicine, Natick, MA 01760-5007.

Brain Research
|September 10, 1993
PubMed
Summary
This summary is machine-generated.

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Hypobaric hypoxia significantly lowered acetylcholine levels in rat hippocampus. Nimodipine treatment reversed these effects, suggesting its protective role against hypoxia-induced cholinergic deficits.

Area of Science:

  • Neuroscience
  • Pharmacology
  • Physiology

Background:

  • Hypoxia is known to decrease acetylcholine (ACh) synthesis and release.
  • The effect of hypoxia on extracellular ACh in vivo remains unclear.
  • Nimodipine, a calcium channel blocker, dilates cerebrovasculature and increases extracellular ACh.

Purpose of the Study:

  • To investigate the impact of hypobaric hypoxia on extracellular ACh and choline levels in the rat hippocampus.
  • To evaluate the protective effects of nimodipine against hypoxia-induced alterations in the cholinergic system.

Main Methods:

  • Microdialysis was employed to measure extracellular ACh and choline in the CA1 and DG regions of the rat hippocampus.
  • Rats were exposed to simulated hypobaric hypoxia (5,500 m) for eight hours.

Related Experiment Videos

  • High-performance liquid chromatography (HPLC) with electrochemical detection was used for dialysate analysis.

    • Main Results:

    • Exposure to 5,500 m significantly reduced extracellular ACh levels compared to sea-level controls.
    • Nimodipine administration reversed the hypoxia-induced decrease in ACh levels.
    • The protective effect of nimodipine was observed with administration before and during hypoxia exposure.

    Conclusions:

    • Hypobaric hypoxia detrimentally affects hippocampal cholinergic function by reducing extracellular ACh.
    • Nimodipine demonstrates a protective effect against hypoxia-induced cholinergic deficits.
    • Nimodipine's protective action may involve its vascular or direct neural effects.