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Updated: Aug 16, 2026

Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina
Published on: October 13, 2014
Glutamate receptor immunoreactivity in the rat suprachiasmatic nucleus
1Circadian Neurobiology Research Group, Armstrong Laboratory/CFTO, Brooks AFB, TX 78235.
Abstract:
Antibodies selective for the glutamate receptor subunits GluR1 and GluR2/3 were used to localize glutamate receptor immunoreactivity in the rat suprachiasmatic nuclei (SCN). These antisera identified two distinct cell populations within the SCN. Cells immunoreactive to GluR2/3 antiserum were located predominantly in the ventral SCN while antiserum selective for GluR1 stained a population located along the dorsal and lateral borders of the nucleus. There were no apparent day-night differences in GluR immunoreactivity observed in the SCN.
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