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Related Experiment Videos

Microsatellite instability in bladder cancer

M Gonzalez-Zulueta1, J M Ruppert, K Tokino

  • 1Kenneth Norris Jr. Comprehensive Cancer Center, University of Southern California School of Medicine, Los Angeles 90033.

Cancer Research
|December 1, 1993
PubMed
Summary

Microsatellite instability was found in early-stage bladder cancers. These genetic changes, affecting DNA repeat lengths, suggest they may play a role in the initial development of transitional cell carcinoma.

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Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • Microsatellite instability (MSI) is a known hallmark of certain cancers.
  • Transitional cell carcinoma (TCC) is the most common type of bladder cancer.

Purpose of the Study:

  • To investigate the presence and characteristics of somatic microsatellite instability in transitional cell carcinomas of the bladder.
  • To determine if MSI occurs early in bladder cancer development.

Main Methods:

  • Analysis of microsatellite repeat lengths in tumor DNA from 200 bladder cancer patients.
  • Examination of specific repeat loci on chromosome 9 and the androgen receptor gene on the X chromosome.
  • Categorization of instability based on number of affected loci and alteration size.

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Main Results:

  • Somatic microsatellite instability was detected in 6 out of 200 bladder TCCs.
  • Instabilities involved (GT)n repeats on chromosome 9 and (CAG)n repeats in the androgen receptor gene.
  • All six tumors with MSI were low stage (Ta-T1), indicating early occurrence.

Conclusions:

  • Microsatellite instability is present in a subset of early-stage bladder cancers.
  • These findings suggest that MSI may be an early event in bladder tumorigenesis.
  • Further research into the role of MSI in bladder cancer progression is warranted.