Related Experiment Videos
Characterization of a polyclonal antiserum against the purified human recombinant calcium binding protein calretinin
B Schwaller1, P Buchwald, I Blümcke
1Institute of Histology and General Embryology, University of Fribourg, Switzerland.
Cell Calcium
|October 1, 1993
Abstract:
We have purified recombinant human calretinin (CR) from Escherichia coli lysates and have produced a polyclonal antiserum against it. The antiserum recognizes determinants conserved in fish, chicken, rat, monkey and human CR. We show its use in the qualitative detection of CR by different methods of immunohistochemistry as well as in the detection of CR on immunoblots.