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Updated: May 1, 2026

Biochemical Titration of Glycogen In vitro
Published on: November 25, 2013
[Regulation of glycogenolysis against hormones in isolated rat hepatocytes]
1Institute of Oncology, Beijing.
Abstract:
cAMP and Ca(2+)-dependent glycogenolytic pathways are regulated by different hormones involved in the activation of glycogen phosphorylase in isolated rat hepatocytes. Dose-dependent glucagon activation of glycogen phosphorylase and lowering of cAMP levels were obtained by vasopressin and angiotensin treatment. Similar time courses of Ca2+ fluxes and phosphorylase activation were induced by vasopressin, angiotensin and phenylephrine. Heterologous down-regulation of hormones was induced in glycogenolysis in hepatocytes. These results could be of significance in physiology studies in vivo.
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