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Serotonin secretion from rat Leydig cells

J C Tinajero1, A Fabbri, D R Ciocca

  • 1Section on Molecular Endocrinology, National Institute of Child Health and Human Development, National Institute of Health, Bethesda, MD 20892.

Endocrinology
|December 1, 1993
PubMed
Summary
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Rat Leydig cells secrete serotonin (5HT), a process stimulated by human chorionic gonadotropin (hCG) primarily via cyclic AMP (cAMP). This finding sheds light on the intricate regulation of testicular function.

Area of Science:

  • Endocrinology
  • Reproductive Biology
  • Cell Signaling

Background:

  • Serotonin (5HT) in rat Leydig cells inhibits gonadotropin-induced cAMP and androgen production via 5HT2 receptors.
  • The regulation of 5HT secretion by Leydig cells remains largely undefined.

Purpose of the Study:

  • To investigate the regulation of serotonin (5HT) secretion in cultured rat Leydig cells.
  • To elucidate the signaling pathways involved in gonadotropin-stimulated 5HT release.

Main Methods:

  • Primary adult rat Leydig cell cultures were used.
  • Serotonin (5HT) release was measured following stimulation with hCG, forskolin, TPA, 8-Br cAMP, and ionomycin.
  • Extracellular calcium dependency was assessed.
  • Immunohistochemistry was employed to localize 5HT in rat testis.

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Main Results:

  • Leydig cells secreted significant amounts of 5HT (100-150 pg/10^6 cells/10 min).
  • Human chorionic gonadotropin (hCG) acutely stimulated 5HT release in a dose-dependent manner, primarily through a cAMP-mediated mechanism, as evidenced by forskolin and 8-Br cAMP stimulation.
  • While hCG-induced release was largely independent of extracellular calcium, ionomycin demonstrated that elevated intracellular calcium can also stimulate 5HT secretion.
  • Leydig cell 5HT content decreased upon stimulation of release, and immunohistochemistry confirmed 5HT presence in interstitial cells of the adult rat testis.

Conclusions:

  • Rat Leydig cells synthesize and secrete serotonin (5HT).
  • Gonadotropin (hCG) stimulates 5HT release predominantly via a cAMP-dependent pathway.
  • Calcium signaling also plays a role in mediating 5HT secretion from Leydig cells.