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Transcription in vitro promoted by the Agrobacterium VirG protein
1Laboratory of Molecular Genetics, Institute for Chemical Research, Kyoto University, Japan.
FEBS Letters
|November 22, 1993
Abstract:
Expression of the virulence genes (vir) on the hairy-root-inducing plasmid pRiA4 is induced by plant signals in Agrobacterium cells through a two-component regulatory system, the VirA-VirG system. We constructed an in vitro transcription system that consisted of the purified VirG protein and the Agrobacterium RNA polymerase holoenzyme. Both versions of VirG, the non-phosphorylated form and the VirA-phosphorylated form, were active but showed different patterns of the pH-dependency for transcriptional activation.