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The Escherichia coli cysG gene encodes the multifunctional protein, siroheme synthase
J B Spencer1, N J Stolowich, C A Roessner
1Department of Chemistry, Texas A&M University, College Station 77843-3255.
Abstract:
Previously, the E. coli cysG gene product had been shown to sequentially methylate uro'gen III to produce precorrin-2, hence it was given the trivial name uro'gen III methylase. We now report that in addition to methylase activity, the CysG protein catalyses both the NAD+ dependent oxidation of precorrin-2 to sirohydrochlorin, but also the insertion of iron into this oxidized intermediate, thereby producing siroheme. Thus CysG is a multifunctional protein solely responsible for siroheme synthesis from uro'gen III in E. coli, and accordingly is renamed siroheme synthase.
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