Cell-associated hemagglutinin of classical vibrio cholerae O1 with reference to intestinal adhesion

Vibrio cholerae O1 86B3 biovar cholerae has at least two types of cell-associated hemagglutinin. One is cell wall-associated and L-fucose sensitive, whereas the other is pili-associated and D-mannose sensitive. A pilus rich variant of 86B3 and a poorly piliated parent strain adhered equally to the rabbit intestine. This adhesion was inhibited by L-fucose but not by D-mannose. A Fab fraction prepared from anti-pilus antibody did not inhibit the adhesion. These results suggest that not the pili but a colonization factor located in the outer membrane of the organisms plays a role in intestinal adhesion.