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Extracellular glycoprotein specific for Saccharomyces sensu stricto
S V Kononova1, A B Tsiomenko, W I Golubev
1Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino.
FEMS Microbiology Letters
|October 1, 1993
Summary
Researchers identified immunohomologic proteins in various Saccharomyces yeast species using antibodies against Saccharomyces cerevisiae mannoprotein (gp400). These proteins were detected in related species but not in other genera, aiding yeast classification.
Area of Science:
- Microbiology
- Yeast Genetics
- Immunology
Background:
- Extracellular mannoproteins are key components of yeast cell walls.
- Saccharomyces cerevisiae mannoprotein (gp400) is a well-characterized cell surface protein.
- Immunological methods can be used to identify homologous proteins across species.
Purpose of the Study:
- To investigate the presence of immunohomologic proteins to Saccharomyces cerevisiae mannoprotein (gp400) in other yeast species.
- To utilize cross-reactivity of antibodies for yeast classification and phylogenetic analysis.
Main Methods:
- Production of affinity-purified rabbit polyclonal antibodies against Saccharomyces cerevisiae mannoprotein (gp400).
- Immunological detection of homologous proteins in culture media of various Saccharomyces and related yeast species using Western blotting or similar techniques.
- Analysis of electrophoretic mobility of detected proteins.
Main Results:
- Immunohomologic proteins with similar electrophoretic mobility to gp400 were found in Saccharomyces bayanus, S. paradoxus, and S. pastorianus.
- Cross-reactive proteins with different electrophoretic behaviors were detected in S. dairensis, S. exiguus, S. kluyveri, S. unisporus, and species reclassified into genera like Arxiozyma, Kluyveromyces, Pachytichospora, Torulaspora, and Zygosaccharomyces.
- These proteins were absent in ascosporous yeasts from other genera.
Conclusions:
- The study demonstrates the presence of conserved extracellular mannoproteins across several Saccharomyces and related yeast species.
- Antibody cross-reactivity serves as a valuable tool for distinguishing and classifying yeast species, particularly within the Saccharomyces complex.
- The findings support the utility of mannoprotein immunodetection in yeast taxonomy and evolutionary studies.