Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Codon preference in corynebacteria

M Malumbres1, J A Gil, J F Martín

  • 1Department of Ecology, Genetics and Microbiology, Faculty of Biology, University of León, Spain.

Gene
|November 30, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inpatient surgical treatment of paediatric proximal humerus fractures between 2000 and 2012.

Journal of children's orthopaedics·2018
Same author

Increasing rates of surgical treatment for paediatric diaphyseal forearm fractures: a National Database Study from 2000 to 2012.

Journal of children's orthopaedics·2017
Same author

Comparative mathematical modelling of a green approach for bioaccumulation of cobalt from wastewater.

Environmental science and pollution research international·2016
Same author

Indications and anatomic landmarks for the application of lower extremity traction: a review.

European journal of trauma and emergency surgery : official publication of the European Trauma Society·2016
Same author

Choosing the best image processing method for masticatory performance assessment when using two-coloured specimens.

Journal of oral rehabilitation·2016
Same author

The present Pyrenean population of bearded vulture (Gypaetus barbatus): its genetic characteristics.

Journal of biosciences·2012
Same journal

TBX6 promotes proliferation, invasion, and migration in colorectal cancer: Integrated transcriptomic and protein interaction network analysis.

Gene·2026
Same journal

Face/off: phase-specific modeling of lineage plasticity using near-patient models in genitourinary cancers.

Gene·2026
Same journal

Hierarchical analysis of metabolic phenotype reveals distinct microbiota and circulatory transcriptome in metabolic dysfunction-associated steatotic liver disease.

Gene·2026
Same journal

Mutation T71R enhanced the structural stability and functional activity of wild type superoxide dismutase cloned from soil metagenome.

Gene·2026
Same journal

Reduced ATXN1 expression as an adverse prognostic indicator in Acute myeloid leukemia.

Gene·2026
Same journal

Constructing regulatory networks of Rubisco post-translational modifications: a novel avenue for engineering environment adaptive plants.

Gene·2026
See all related articles

Codon usage analysis in Brevibacterium and Corynebacterium species reveals shared characteristics due to close relatedness. These findings aid in identifying protein-coding regions and designing genetic probes.

Area of Science:

  • * Molecular Biology
  • * Genomics
  • * Microbial Genetics

Background:

  • * Comparative analysis of codon usage (CU) in closely related bacteria.
  • * Investigation of G+C content variations in coding and non-coding regions.
  • * Exploration of mutation pressure and its impact on gene sequences.

Purpose of the Study:

  • * To analyze and compare codon usage patterns in Brevibacterium lactofermentum and Corynebacterium glutamicum (BLCG).
  • * To identify preferred codons and assess their relationship with gene expression.
  • * To compare codon usage in corynebacteria with other bacterial species like Streptomyces and Escherichia coli.

Main Methods:

  • * Analysis of codon usage (CU) and synonymous codon usage (SCU) for 34 BLCG genes and 23 other corynebacterial genes.

Related Experiment Videos

  • * Determination of G+C content in coding and non-coding regions, and ribosomal RNA (rRNA) sequences.
  • * Calculation of the effective number of codons (Nc) to assess GC3s dependence.
  • * Comparison of preferred codons across different bacterial species.

    • Main Results:

    • * BLCG genes exhibit G+C content ranging from 50-62%, with non-coding regions showing lower G+C content than coding regions and rRNA.
    • * A set of 25 preferred codons were identified for highly expressed genes in the BLCG group.
    • * Codon usage in corynebacteria shows greater similarity to Streptomyces than to Escherichia coli.
    • * Effective number of codons (Nc) values differed between the BLCG group and other corynebacterial sequences.

    Conclusions:

    • * Codon usage patterns reflect the close evolutionary relationship between B. lactofermentum and C. glutamicum.
    • * Identified preferred codons can be used to infer gene expression levels.
    • * Codon usage tables are valuable tools for identifying protein-coding regions and designing oligonucleotide probes.