Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cellularly generated active oxygen species and HeLa cell proliferation

R H Burdon1, V Gill

  • 1Department of Bioscience & Biotechnology, Todd Centre, University of Strathclyde, Glasgow, Scotland, UK.

Free Radical Research Communications
|January 1, 1993
PubMed
Summary

Low levels of active oxygen species, like hydrogen peroxide and superoxide, act as crucial cell growth signals in HeLa cells. This suggests a novel redox regulatory system influencing cell proliferation pathways.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dabrafenib and Trametinib Therapy for Advanced Anaplastic Thyroid Cancer - Real-World Outcomes From UK Centres.

Clinical oncology (Royal College of Radiologists (Great Britain))·2022
Same author

Differentiated Thyroid Cancer in Children: A UK Multicentre Review and Review of the Literature.

Clinical oncology (Royal College of Radiologists (Great Britain))·2019
Same author

Experiences of discrimination among people using mental health services in England 2008-2011.

The British journal of psychiatry. Supplement·2013
Same author

Preliminary evidence for biologic activity of toceranib phosphate (Palladia(®)) in solid tumours.

Veterinary and comparative oncology·2012
Same author

Thyroxine dosing after I131Radioiodine ablation for differentiated thyroid cancer.

Clinical oncology (Royal College of Radiologists (Great Britain))·2011
Same author

Reply to the editorial on the HiLo trial.

Clinical oncology (Royal College of Radiologists (Great Britain))·2008

Area of Science:

  • Cell Biology
  • Biochemistry
  • Redox Signaling

Background:

  • Cell growth is regulated by complex signaling pathways.
  • Active oxygen species (AOS) roles in cellular processes are increasingly recognized.
  • The specific function of AOS in HeLa cell proliferation requires further elucidation.

Purpose of the Study:

  • To investigate the role of active oxygen species, specifically hydrogen peroxide and superoxide, as growth regulatory signals in HeLa cells.
  • To determine if AOS constitute a novel redox regulatory system controlling cell growth.
  • To explore the involvement of both intracellularly and extracellularly generated superoxide in HeLa cell growth.

Main Methods:

  • HeLa cells were treated with exogenous hydrogen peroxide.

Related Experiment Videos

  • Experiments utilized superoxide dismutase (SOD), SOD mimics, and inhibitors of SOD and xanthine oxidase.
  • Cellular responses to varying levels of AOS were monitored.

    • Main Results:

    • Low levels of hydrogen peroxide and superoxide were identified as significant growth regulatory signals in HeLa cells.
    • Evidence suggests a novel redox control system superimposed on existing signal transduction pathways.
    • Both intracellular and extracellular superoxide, along with cellularly generated hydrogen peroxide, were found to be important for growth control.

    Conclusions:

    • Active oxygen species function as critical growth regulatory signals in HeLa cells.
    • A novel redox regulatory system involving superoxide and hydrogen peroxide likely modulates cell proliferation.
    • Targeting AOS pathways may offer new strategies for controlling cell growth.